Maize 4114 produces three insecticidal proteins classified as Cry1F, Cry34Ab1 and Cry35Ab1, as well as the PAT protein that confers tolerance to the active herbicide ingredient glufosinate-ammonium. It was genetically engineered using Agrobacterium tumefaciens.

Regulation (EU) No 503/2013, which foresees 90-day animal feeding studies, an extended literature review, specific monitoring requirements and specific statistical analysis was applied in the risk assessment of maize 4114.